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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has become noticed in lots of tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was found to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a critical function in advertising and marketing mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are explained. Two further splice variants were being documented but detailed nucleotide sequence information and facts is just not yet out there.

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Blocking peptides are peptides that bind exclusively to the target antibody and block antibody binding. These peptide typically is made up of the epitope identified by the antibody.

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This manual outlines additional possible optimization of mobile and immunoassay parameters to be sure the best possible final results are...

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This manual outlines further more possible optimization of mobile and immunoassay parameters to be certain the best possible final results are...

Antibodies sure to the blocking peptide no longer bind into the epitope within the focus on protein. By comparing the staining in the blocked antibody versus the antibody by yourself, one can see which staining is precise.

Concentrate on Details Cyclin E belongs to the remarkably conserved cyclin relatives, whose associates are characterized by a spectacular periodicity in protein abundance throughout the mobile cycle. Cyclins functionality as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of each mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S transition. This protein accumulates on the G1-S phase boundary and is particularly degraded as cells progress via S phase.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...

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